Journal: The Journal of Biological Chemistry

Article Title: Human Memory, but Not Naive, CD4 + T Cells Expressing Transcription Factor T-bet Might Drive Rapid Cytokine Production *

doi: 10.1074/jbc.M114.608745

Figure Lengend Snippet: Kinetics of cytokine secretion by activated naive and memory CD4+ T cells. Naive and memory CD4+ T cells were isolated from CD4+ T cells by MACS microbeads, and the purity of naive or memory CD4+ T cells was more than 97% as determined by flow cytometry (a). Naive and memory CD4+ T cells were stimulated with (open histogram) or without (shaded histogram) PMA and ionomycin in the presence of BFA for 6 h. Cells were stained by intracellular staining and analyzed by FACS (b). Purified naive and memory CD4+ T cells were stimulated with immobilized anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 0 to 72 h, and the concentrations of cytokines IFN-γ, IL-2, and TNF-α were detected by ELISA (c). Data are representative of three separate experiments with similar results.

Article Snippet: Human ELISA kits for cytokines IFN-γ, IL-2, and TNF-α were purchased from BD Biosciences.

Techniques: Isolation, Flow Cytometry, Staining, Purification, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: The levels of serum IL-31 were assessed in patients with asthma (n = 44) and controls (n = 22) as measured by ELISA. ( A ) Serum IL-31 was increased in patients with asthma compared with the controls; ( B ) When patients were stratified according to the status of atopy, the serum IL-31 levels in patients with allergic asthma (n = 23) were higher than those in patients with nonallergic asthma (n = 21) and controls; ( C ) Serum IL-31 was increased in severe asthma patients (n = 22) compared with mild asthma patients (n = 22) and controls; ( D ) Receiver operating characteristic (ROC) curve for distinguishing patients with asthma from the controls. The area under the ROC curve was 0.791 (95% CI: 0.66 to 0.86). Data are presented as median with interquartile range. *p < 0.05, ***p < 0.001.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: ( A ) BALF samples were measured by ELISA and the BALF IL-31 levels in patients with asthma (n = 9) were significantly increased compared with the controls (n = 5); ( B ) Spearman’s rank correlation analysis showed the serum IL-31 levels correlated positively with BALF IL-31 levels ( r = 0.63; p = 0.016). Data are presented as median with interquartile range. **p < 0.01.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Interleukin-31 expression and relation to disease severity in human asthma

doi: 10.1038/srep22835

Figure Lengend Snippet: The classical Th2 cytokines such as IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were further measured by ELISA. The levels of serum IL-5, IL-13 and TSLP were significantly higher in patients with asthma (n = 44) compared with control subjects (n = 22) ( A–C ). Spearman’s rank correlation analysis showed a significant correlation between the serum IL-31 levels and the serum IL-5 levels ( r = 0.34, p = 0.024), the serum IL-13 levels ( r = 0.43, p = 0.003), the serum TSLP levels ( r = 0.42, p = 0.004) ( D–F ). Data are presented as median with interquartile range. **p < 0.01, ***p < 0.001.

Article Snippet: The levels of IL-31, IL-5, IL-13 and thymic stromal lymphopoietin (TSLP) were assessed by ELISA, using ELISA Kits for the assay of human IL-31, IL-5, IL-13 and TSLP (Uscn Life Science Inc. Wuhan) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: OE elevated the levels of IL-6 (A) and IL-8 (B) in BEAS-2B cell culture media. Cells were exposed to OE at indicated concentration for 16 h. Values represent means ± SEM, n = 3, * p < 0.05 compared to the respective control.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Cell Culture, Concentration Assay

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: Inhibition of OE-induced IL-6 and IL-8 production by NAC and SFN. BEAS-2B cells were pre-incubated with or without NAC or SFN for 2 h before exposure to OE for 16 h. (A and B) Effects of NAC and SFN on IL-6. (C and D) Effects of NAC and SFN on IL-8. Inset: Induction of HO-1 expression by SFN after a 6-h exposure. OE50: OE at 50 μg/ml. Values represent means ± SEM, n = 3. * p < 0.05 compared to the respective control; ** p < 0.05 compared to OE without NAC.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Inhibition, Incubation, Expressing

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: The role of p38 in mediating the pro-inflammatory effect of OE. (A) Increased p38 phosphorylation in OE-treated BEAS-2B cells. Cells were exposed to OE for 2 and 4 h. Total p38 was used as internal control. (B) Effect of p38 inhibitor on IL-6 production by OE-treated cells. (C). Effect of p38 inhibitor on OE-induced IL-8 production. BEAS-2B cells were pre-incubated with p38 inhibitor SB203580 for 2 h prior to being exposed to OE for 16 h. OE50: OE at 50 μg/ml. Values represent means ± SEM, n = 3, * p < 0.05 compared to the respective control; ** p < 0.05 compared to OE alone.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Incubation